Small molecule overproduction for high value chemicals is a current target for translational research with high throughput screening emerging as a defining tool in the synthetic biology reformation. TraDISort’n’Seq is the union of Next-Gen sequencing of transposon mutants with high throughput screening of cell properties. Cells in a TraDIS library can be sorted using physical and chemical characteristics, in the order of tens of thousands in minutes. It has been hypothesised that by incorporating a fluorescent reporter, within a TraDIS Library, mutants that show differential production of a target ligand can be identified using fluorescent activated cell sorting. This will provide a metabolic map of proteins of interest across the whole metabolome. We have designed a plug and play biosensor for use with TraDISort’n’Seq. Strategies for copy number and genome integration are explored with ratio-metric normalisation using a secondary fluorescent protein. The overproduction of polyamines in several E. coli strains is being explored with this method. Polyamines are precursors to bioplastics that have improved material properties in comparison to those derived from fossil fuels. With iterative rounds of TraDISort’n’Seq, guided holistic genetic engineering will reduce the time for complex strain construction for small chemical overproduction.