The association between waterborne disease outbreaks and public aquatic facilities, such as swimming pools, is well established. Water play parks are a new type of public aquatic facility emerging across Australia and the world. Previous research has identified the microbial risks associated with public swimming pools however similar risks have only been investigated to a limited extent in water play parks. Although outbreaks have been associated with these parks, there is limited empirical evidence of the microbial risks associated with these settings. We investigated the bacterial quality of water and surfaces within a water play park on multiple occasions. Using membrane filtration for water samples and the spread plate method for swab samples of surfaces, initial results indicated the presence of bacteria within the water samples and on multiple surfaces within the water play park. Several bacteria were isolated from both water samples and swab samples of surfaces with gram staining indicating the presence of both gram-positive and gram-negative bacteria. Using MALDI-TOF MS, several species of bacteria including Staphylococcus spp., Bacillus spp. and Pseudomonas spp. were identified within this environment. Some of the bacteria identified are opportunistic pathogens and have the potential to cause adverse public health outcomes, especially among immunocompromised individuals or vulnerable groups such as children who are the main users of water play parks. Testing for antibiotic susceptibility and the potential to form biofilms was undertaken on some of the bacterial isolates to identify the potential public health risks of the bacteria isolated from the water play park. It is anticipated that these findings will provide evidence for the types of bacteria that may be present in water play parks and the associated potential public health risks.