Old Wallow is located on the Ingrid Christensen coast of East Antarctic within the Vestfold Hills region. It is an oligotrophic, ice-free, polar desert with minimal previous analysis conducted on the site. This study will investigate the soil microbiome through analysis on taxonomic composition using amplicon sequencing targeting the 16S and 18S rRNA gene sequences. A total of thirty-one bacterial phyla and thirty-four micro-eukaryotic phyla were classified. Dominant bacterial phyla were consistent with other Antarctic terrestrial environments including Actinobacteria, Bacteroidetes and proteobacteria. Interestingly Chloroflexi and Gemmatimonadetes were also dominant representing over 9.8% and 6.6% relative abundance respectively. Micro-eukaryotic diversity was of greater interest with dominant phyla associated with algal communities including Ochrophyta, Chlorophyta and Cercozoa. With limited traditional photosynthesizers found, such as cyanobacteria, algae are uniquely positioned as primary producers within Old Wallow. This can only be assumed using molecular analysis; therefore, further research is required to confirm this hypothesis. Primary production will be analyzed using a variety of techniques including DNA-SIP, gas chromatography and metagenomic classification of micro-eukaryotes. Network analysis on Old Wallow using maximal information coefficients highlighted the presence of numerous microbial clusters that differed in association with biotic and abiotic measures. These microbial clusters were significantly associated with transect location with spatial distribution connected to environmental parameters such as Aluminum oxide, Sodium oxide, and sulphate. Microbial communities within Old Wallow form highly integrated communities with numerous significant connections found between species. Highly integrated communities are incredibly fragile with minor changes in environmental conditions having significant effects on microbial diversity. With the effects of climate change looming, the fate of Antarctic terrestrial environments remains in the balance. Old Wallow presents a unique Antarctic terrestrial site positioned for environmental conservation.