Matrix-assisted laser desorption/ionisation time of flight (MALDI-TOF) has vastly improved identification techniques in laboratories, but has its limitations. Bruker has stated that their Microflex LT MALDI Biotyper has difficulty differentiating Streptoccocus pyogenes, Streptococcus canis and Streptococcus dysgalactiae as they have very similar Mass Spectral Profiles (MSPs). Our research has shown that the correct Streptococcus species was returned as the top score for only 33 of the 40 (82.5%) isolates tested when compared to 16S RNA Sequencing as the gold standard. The 7 isolates incorrectly identified were Group G S.dysgalactiae isolates, of which 6 were incorrectly identified as S.canis and 1 incorrectly identified as S.pyogenes. The aim of this study was to formulate an algorithm that uses Bruker’s Microflex LT MALDI Biotyper for the correct identification of Lancefield Group C and G Streptococci as S.dysgalactiae or S.canis. The algorithm that we have formulated describes that if the same species is listed as the top 3 results with a log score >2, the identity is correct. Following the algorithm, 30 of the 40 (80%) isolates fell into this category and all 30 (100%) were correctly identified when compared to 16S Sequencing. Of the remaining 10 isolates, trehalose acidification was used to differentiate between S.dysgalactiae and S.canis. 9 isolates were trehalose positive which is consistent with S.dysgalactiae. The remaining isolate was trehalose negative, consistent with S.canis. These results were confirmed by 16S RNA sequencing. The next step will be to expand this research by including Lancefield Group A isolates in the algorithm. This study gives us confidence when using MALDI-TOF as the primary means of identification for haemolytic streptococci.