Objectives: Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation, cell surface hydrophobicity (CSH) and hemolysin production are attributes associated with pathogenicity of oral Candida. Candida albicans and Candida dubliniensis are associated in causing oral candidosis. Lysozyme, is renowned for their functionality in regulating the quantity and quality of microflora on mucosal surfaces. Lysozyme, has shown to exert antimicrobial activity on a range of microorganisms in vitro including oral Candida species. Thus, we investigated the impact of lysozyme on adhesion to BEC and DAS, GT formation, CSH and hemolysin production of C. albicans and C. dubliniensis isolates. Materials and Methods: After exposure to lysozyme for 1h, the susceptibility to lysozyme of 20 isolates each of C. albicans and C. dubliniensis isolates were determined following a 48h period of incubation. Candida cell suspensions, obtained from colony forming units after this period, were assessed for adhesion to BEC and DAS, GT formation, CSH, and hemolysin production using in-vitro assays. Results: Anti-fungal activity of lysozyme (CFU ml-1 of control suspension - CFU ml-1 of lysozyme containing suspension/ CFU ml-1 of control suspension) on C. dubliniensis ranged from 0.51 to 0.59 (mean of 0.55) and for C. albicans it ranged from 0.53 to 0.59 (mean of 0.56). Exposure to lysozyme suppressed the ability of C. albicans isolates to adhere to BEC, DAS, GT formation, CSH and hemolysin production by a percentage reduction of 36.14%, 34.12%, 19.87%, 29.57% and 8.11%, respectively, and C. dubliniensis isolates by a percentage reduction of 41.65%, 42.48%, 23.39%, 33.75% and 12.66%, respectively (p <0.01 for all). Conclusion: These data provide a tantalizing glimpse that exposure of C. albicans and C. dubliniensis isolates to lysozyme even for a brief period would induce a sustainable antifungal effect by suppressing adhesion related attributes and hemolysin production of these Candida species in vitro. Acknowledgements: This work was supported and funded by Kuwait University Research Grant No. [DB 01/18].